gms | German Medical Science

International Conference on SARS - one year after the (first) outbreak

08. - 11.05.2004, Lübeck

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Efficient transport of a novel SARS coronavirus protein, U274, to the cell surface

Poster

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  • Yee-Joo Tan - Institute of Molecular and Cell Biology, Singapore
  • Shuo Shen - Institute of Molecular and Cell Biology, Singapore
  • corresponding author presenting/speaker Timothy H.P. Tan - Institute of Molecular and Cell Biology, Singapore
  • Phuay-Yee Goh - Institute of Molecular and Cell Biology, Singapore
  • Burtram Fielding - Institute of Molecular and Cell Biology, Singapore
  • Eng-Eong Ooi - Environmental Health Institute, National Environmental Agency, Singapore
  • Hwee-Cheng Tan - Environmental Health Institute, National Environmental Agency, Singapore
  • Seng Gee Lim - Institute of Molecular and Cell Biology, Singapore
  • Wanjin Hong - Institute of Molecular and Cell Biology, Singapore

International Conference on SARS - one year after the (first) outbreak. Lübeck, 08.-11.05.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04sarsP6.03

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/sars2004/04sars117.shtml

Veröffentlicht: 26. Mai 2004

© 2004 Tan et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

The SARS-CoV genome contains open reading frames (ORFs) that encode for several genes that are homologous to proteins found in all known coronaviruses. These are the replicase gene 1a/1b and the 4 structural proteins, nucleocapsid (N), spike (S), membrane (M) and envelope (E), and these proteins are expected to be essential for the replication of the virus. In addition, it also contains 10 potential ORFs varying in length from 39 to 274 amino acids. The largest among these is the first ORF of the second longest subgenomic RNA and this protein (termed U274 in this study) consists of 274 amino acids and contains 3 putative transmembrane domains. Using antibody specific for the C-terminus of U274, U274 was shown to be expressed in SARS-CoV infected Vero E6 cells. Besides the full-length protein, two other processed forms were also detected. Using direct immunoflurorescence, U274 was localized to the perinuclear region as well as to the plasma membrane, in both transfected and infected cells. A N-terminus myc-tagged U274 was used to confirm the topology of U274 and to show that it is expressed on the surface in fixed and unpermeabilized cells. FACS scan analysis also showed that myc-U274 was expressed on the surface of live cells. In addition, U274 can interact specifically with the M and E proteins, as well as with U122, another protein unique to SARS-CoV. U274 can also undergo self-dimerization.