Artikel
Identification of a new human coronavirus
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Autoren
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Lia van der Hoek
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Krzysztof Pyrc
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Maarten F. Jebbink
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Wilma Vermeulen-Oost
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Ron J.M. Berkhout
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Katja C. Wolthers
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Pauline M.E. Wertheim-Van Dillen
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Jos Kaandorp
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Joke Spaargaren
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
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Ben Berkhout
- Department of Human Retrovirology Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
| Veröffentlicht: | 26. Mai 2004 |
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Gliederung
Text
Three human coronaviruses are known to exist: HCoV-229E, HCoV-OC43 and SARS-CoV. We identified a fourth human coronavirus (HCoV-NL63) using a new virus discovery method. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence demonstrates that this virus is not a recombinant, but a novel group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population.
