Artikel
Diguanosinepentaposphate induces osteopontin ind vsmc
Diguanosinepentaphosphat induziert Osteopontin in VSMC
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Veröffentlicht: | 8. August 2006 |
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Gliederung
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Objective: Cardiovascular disease (CVD) is the most common cause of death in industrialised countries. Dinucleoside polyphosphate could be identified in human platelets. Adenosine-guanosine and diadenosine polyphosphates have increasing blood pressure properties via activation of P2x-receptors. High blood pressure is a major risk factor for atherosclerosis. Osteopontin (OPN) is a phosphoprotein with different regulatory functions including atherosclerosis. The aim of this study was to investigate the effect of dinucleoside polyphosphates on OPN expression in vascular smooth muscle cells (VSMC) as a atherosclerosis promoting factor.
Design and method: VSMC were stimulated with different concentrations of diguanosine pentaphosphate (Gp5G) for 6 hours. As a positive control UTP was used. VSMC were harvested, total RNA was isolated and OPN mRNA levels were quantified by real-time-PCR.
Results: Gp5G induces OPN in a dose-dependent manner. 100nmol/l Gp5G induces a 2,67±0,09-fold OPN expression versus unstimulated VSMC.
Conclusions: Dinucleosidpolyphosphates are well known to have vasoactive properties. These data show, that Gp5G as a member of dinucleoside poylyphosphates has OPN stimulation properties. Thus, Gp5G might play a role in formation of atherosclerotic plaques via OPN induction.