Artikel
Expression of MMP-3, TIMP-1, IL-1beta and TGF-beta1 after IL-4 treatment in CVB-3-induced myocarditis in BALB/c mice
Expression von MMP-3, TIMP-1, IL-1beta und TGF-beta1 nach IL-4 Behandlung in CVB3-induzierter Myokarditis in BALB/c-Mäusen
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Autoren
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S. Leschka
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S. Rutschow
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K. Puhl
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P. Schwimmbeck
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C. Tschöpe
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M. Pauschinger
| Veröffentlicht: | 11. November 2004 |
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Gliederung
Text
Serveral proinflammatory and anti-inflammatory cytokines are critically involved in determining the course of viral myocarditis, and immunomodulation with different cytokines may have either deleterious or protectiv effects. Following acute CVB3 infection, intramyocardial inflammation is associated with altered myocardial matrix matalloproteinase/tissue inhibitor of MMP (MMP/TIMP) expression and left ventricular dysfunction. In this study we evaluated inflammation, cytokine and MMP/TIMP profiles, and left ventricular function in responce to IL-4 treatment in CVB3 induced akute murin myocarditis.
10 weeks old male BALB/c mice are randomized in 5 groups: control, infected and treatment with IL-4 i.p. in the early (first 5 days, EAP), late (last 5 days, LAP) and continuous acute phase (CAP). The infection was performed by intraperitoneally injection of 1,5x10E5 PFU CVB3. In all mice hemodynamic function (dP/dt max) was evaluated by invasive measurement (tip catheter). In addition relevant cytokines for myocardial remodeling (IL-1beta, TGF-beta1) and matrix active enzymes (MMP-3, TIMP-1) were analyzed by semiquantitative RT-PCR.
Early IL-4 administration in akute viral myocarditis resulted in significantly improved left ventricular function. The beneficial effects of exogenous IL-4 on LV dysfunction in acute myocarditis may be due to limitation of intramyocardial inflammation and restouration of MMP/TIMP ratio [Tab. 1]. Further studies on IL-4 and IL-4-inducing drugs may offer new therapeutic approach in viral myocarditis.
