Artikel
Vasoactive peptides in the human placenta: impact on the arachidonic acid signaling
Vasoaktive Peptide in der humanen Plazenta: Einfluß auf die Arachidonsäuresignalübertragung
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Autoren
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A. Jank
- Freie Universität Berlin, Universität Leipzig (Berlin, Leipzig, D)
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F. Gembardt
- Freie Universität Berlin, Universität Leipzig (Berlin, Leipzig, D)
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H. Stepan
- Freie Universität Berlin, Universität Leipzig (Berlin, Leipzig, D)
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H. Hopp
- Freie Universität Berlin, Universität Leipzig (Berlin, Leipzig, D)
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T. Walther
- Freie Universität Berlin, Universität Leipzig (Berlin, Leipzig, D)
| Veröffentlicht: | 11. November 2004 |
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Gliederung
Text
The placenta is thought to play an important role in the aetiology and pathogenesis of preeclampsia. This clinical syndrome of hypertension, proteinuria and peripheral/cerebral oedema occurs after the 20th week in 3-5% of all pregnancies. The detection of arachidonic acid (AA) release in the placenta observes the basic step in the cyclooxygenase pathway and could offer wide information about the relevance of vasoactive peptides in placental vascular reactivity and inflammation. Therefore the aim of this study was to investigate the role of specific vasoactive peptides for the intracellular signaling in primary human placental cells.
Placental tissue samples of 6 normal and 3 preeclamptic pregnancies at term were prepared for cell culture using a trypsin digestion. 72 hours after first plating the H3-AA-prelabelled placental cells were stimulated by angiotensin II, angiotensin-(1-7), oxytocin and bradykinin (10-10-10-6M) and the AA release was measured.
Preparation of placental tissue for primary cell experiments was successfully established. None of the 4 tested peptides increased the AA release in placental cells of normal pregnancies. Preliminary data with primary placental cells of 3 preeclamptic pregnancies suggest no rise in AA release after stimulation with all tested peptides.
Our data implicate that the tested peptides mediate no enhanced AA release in placental cells. However the measurement of the released AA in primary cell culture of human placenta could be a helpful tool for defining the altered peptide actions in preeclamptic placentas. Further experiments with this assay could identify deregulated signaling for other circulating peptides in placentas with defined pathology.
