Artikel
Differential expression of laminins in salivary glands and associated tumors
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Autoren
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Julia Dlugaiczyk
- Klinik und Poliklinik für Hals-, Nasen-, Ohrenkranke, Erlangen
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Johannes Zenk
- Klinik und Poliklinik für Hals-, Nasen-, Ohrenkranke, Erlangen
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Heinrich Iro
- Klinik und Poliklinik für Hals-, Nasen-, Ohrenkranke, Erlangen
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Olaf Wendler
- Klinik und Poliklinik für Hals-, Nasen-, Ohrenkranke, Erlangen
| Veröffentlicht: | 22. September 2005 |
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Gliederung
Text
Laminins belong to a family of heterotrimeric glycoproteins, which are assembled of α-, β- and γ-subunits, respectively. They promote cell differentiation, adhesion and migration within the extracellular matrix (ECM). Recently, laminins - especially laminin 5 (α3 β3 γ2) - have been described as markers of tumor invasion and metastasis.
The aim of our study is to investigate into the role of laminins in growth and recurrency of parotid gland tumors.
Differential expression of all published laminin isoforms in normal parotid tissue was studied with immunofluorecence. α2-, α4- and γ1-chains showed a spatial association with the acinar basement membrane, whereas α3-, β2- and γ2-chains were predominantly expressed in ductal basement membranes. α5- and β1-isoforms were detected in both acini and ducts. We observed an up-regulation of laminin 5 in pleomorphic adenoma with immunofluorescence. Furthermore, the β2-chain, which was only faintly expressed in normal tissue, produced an intense staining for most parotid tumors.
Oligo-microarrays revealed different expression profiles of laminin transcripts in normal parotid tissue and pleomorphic adenoma.
Our present results demonstrate an altered expression pattern of laminin isoforms in parotid tumors compared to normal tissue on both transcript and protein level.
In further experiments, the role of laminins in cell adhesion and migration of parotid tumor cells is to be studied in cultured cell lines.
