Artikel
Identification and classification of gliomas by optical spectroscopic imaging techniques
Identifizierung und Klassifizierung von Gliomen durch optische spektroskopische Imagingtechniken
Suche in Medline nach
Autoren
Veröffentlicht: | 30. Mai 2008 |
---|
Gliederung
Text
Objective: Optical spectroscopic imaging techniques have the potential to expand the ability to diagnose tissue beyond that of standard pathological methods because they characterize tissue at a microstructural molecular level with a spatial resolution in the single cell range. With the aim to achieve an intraoperative glioma grading, infrared spectroscopy was used to characterize and classify specimens of human glioma patients according to the grade of malignancy.
Methods: Spectroscopic maps of 54 native specimens of 6 patients with human gliomas were collected ex vivo using Neuronavigation guidance and were analyzed by infrared (IR) spectroscopy. All glioma patients had inhomogeneous tumors showing regions of different tumor grades. Variations of tissue composition within each spectroscopic map were demonstrated by a linear discriminant analysis classification model and were compared to histopathological and neuroradiological findings.
Results: Normal tissue and low-grade or high-grade regions of glioma could be reliably distinguished by IR spectroscopy. A sensitivity of up to 100% (22/22) and a specifity of up to 96,9% (31/32) was achieved in distinguishing low-grade from high grade glioma probes.
Conclusions: Infrared spectroscopic imaging has a great potential for intraoperative identification and grading of cerebral glioma.