Artikel
Differential gene expression in adult neural progenitor cells from the human dentate gyrus and olfactory bulb
Differentielle Genexpression in adulten neuralen Vorläuferzellen aus dem humanen Gyrus dentatus und Bulbus olfactorius
Suche in Medline nach
Autoren
Veröffentlicht: | 23. April 2004 |
---|
Gliederung
Text
Objective
In the last few years, several neurogenic regions have been identified in the adult brain of rodents and humans, of those being most important the dentate gyrus (DG) belonging to the hippocampal region and the so-called rostral migratory pathway leading from the subventricular zone of the lateral ventricles to the olfactory bulb (BO). However, little is known about the differential characteristics of neural progenitor cells coming from either one of these two major neurogenic regions. Therefore the aim of the present study was to identify genes being differentially expressed in the dentate gyrus and the olfactory bulb of the adult human brain, by using mRNA microarray analysis.
Methods
Neural progenitor cell cultures were established from the dentate gyrus and the olfactory bulb of adult patients undergoing neurosurgical operations. Expression of various differentiation markers was demonstrated by immunohistochemistry and RT-PCR. Furthermore, RNA of early and advanced passages was subjected to mRNA microarray analysis.
Results
Primary cultures of adult neural progenitor cells isolated from the human dentate gyrus (DG) and the human olfactory bulb (BO) expressed the neural stem cell marker nestin as well as markers of the neuronal and glial cell lineage on protein and on mRNA level. Although these cells showed a similar expression of the markers analysed, mRNA microarray analysis comparing DG cells and BO cells revealed differential expression of a number of genes. Most of them are associated with a high proliferation capacity and, interestingly, were overexpressed in the DG cell lineage.
Conclusions
Our data suggest that adult neural progenitor cells from the dentate gyrus might have a higher proliferation potential. This can be of importance for future therapeutic applications.