Figure 1: B cell numbers and characteristics at baseline in RTX treated patients
A: Frequency and absolute numbers of CD19+ B cells in IgG seroconverted RTX patients (RTX IgG+) compared to vaccination non-
responder (RTX IgG-) 7 days after 2nd vaccination. Color code: previously infected individuals are indicated as red quadrats; 2x vaccinated
with ChAdOx1 indicated in green; 2x heterologous vaccinated 1x ChAdOx1 followed by 1x BNT162b2, indicated in blue.
B: Distribution of B lineage cell subsets before vaccination in HD, RA, RTX IgG+ and RTX IgG- study participants.
C: Spearman's correlation matrix showing relation between humoral responses, absolute cell counts and expression of CXCR5, HLA-DR, CD95, CD21low, PD-1, PD-L1 on B cells before vaccination. Corresponding correlations are represented by red (negative) or blue (positive) circles; size and intensity of color refer to the strength of correlation. Only correlations with p s 0.01 are indicated. Values in circles indicates r value of correlation. DN
“double negative” CD27-lgD- B cells. PB plasmablast.