Figure 6: Indirect fluorescence in situ hybridization (FISH): primate retinal tissue containing antigens very similar to human retinal tissue was incubated with patient serum. After a wash off procedure, human IgG antibodies that bind fluorochrome were added to detect anti-retinal antibodies with fluorescence microscope. Anti-retinal autoantibodies against the inner nuclear layer (INL), outer plexiform layer (OPL) and photoreceptor layer (PhotoR) were detected.