gms | German Medical Science

Figure

Figure 1: Gating strategy used for flow cytometry.
Lymphocytes were gated by forward and side scatter and single cells were identified by a lower FSC-W. CD11b+ myeloid cells were gated based on CD11b positivity and macrophages were identified as CD11b+F4/80+SiglecF, eosinophils as CD11b+F4/80lowSiglecF+, monocytes as CD11b+Ly6C+Ly6G and neutrophils as CD11b+Ly6C+Ly6G+ cells. Alternatively activated macrophages were identified as RELMα positive based on the fluorescence minus one approach.