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Figure

Figure 2: TGFβ depletion does neither alter E. coli-induced hypothermia, peritoneal bacterial burden, nor impair the filaria-mediated protective effect.
(A) Kinetic of the body temperature after intraperitoneal injection of 8.5x108–1.0x109 cfu E. coli K12 in chronic L. sigmodontis (L.s.)-infected BALB/c mice (L.s. Isotype: n=20; L.s. anti-TGFβ: n=20) and uninfected controls that received either anti-TGFβ or an isotype control before E. coli challenge (Isotype: n=18; anti-TGFβ: n=17. (B) Peritoneal bacterial load [cfu], (C) absolute cell number of CD11b+F4/80+ macrophages as well as (D) the frequency of macrophages expressing RELMα and macrophage mean fluorescence intensity (MFI) of (E) RELMα and (F) CD86 six hours after E. coli injection. Pooled data of three independent experiments with at least 6 mice per group. Data is shown as median (A), box and whisker blots with min/max (B–F) and was tested for statistical significance by 2-way ANOVA and Bonferroni post hoc test (A), Kruskal-Wallis test and Dunn’s Multiple Comparison post hoc test (B,C,E–F) or 1-way ANOVA with Tukey’s multiple comparisons test (D).