Figure 4: Cytotoxic effects of chaetocin on cell viability of generated CIK cells. The cells were incubated in medium containing 0–10 nM chaetocin and the cell viability was determined after 1 day (red), after 3 days (green) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ąSD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.