gms | German Medical Science

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Figure 4: Quantitative real-time RT-PCR analysis of MMP-1 mRNA expression after single wIRA(+RL) or UV-A(+BL) irradiation at 30C

An irradiation dose response assessment was performed on human dermal fibroblast cells with wIRA(+RL) irradiation doses of 340, 680, 1020 and 1360 J/cm respectively (corresponding to 300, 600, 900 and 1200 J/cm wIRA and corresponding to 15, 30, 45 and 60 minutes irradiation) performed on a 30C temperature regulated water bath. Alternatively UV-A(+BL) irradiation (50 J/cm, corresponding to 30 minutes irradiation) was used. Data represent the average of five experiments with the associated standard deviation of the mean. Fibroblasts were harvested 24h after irradiation to assess MMP-1 mRNA expression by quantitative real-time RT-PCR as described in the text. Data were analyzed with the 2-ΔΔCT method [43], results are given as relative MMP-1 mRNA expression normalized to the actin mRNA expression.