Figure 4: HPLC-ESI-MS chromatogram of a digested haemolysate sample obtained by using the optimized chromatographic conditions with column-batch 2.
Overlaid graphs: mass trace of the doubly charged β-N-terminal hexapeptide of HbA 0 (m/z 348.3 amu, blue line) and HbA 1c (m/z 429.3 amu, red line).