Figure 3: A representative 2.5D microscopy image of the biofilm disruption and inactivation of P. aeruginosa biofilm following a 60 minute contact time (n=1). The untreated control (top) demonstrates the abundance of live/viable bacterial cells, as indicated by the green signal, forming microcolonies across the surface, whilst the sample treated with PHMB-poloxamer (bottom) reveal a reduced cell viability (no green signal, little yellow signal as overlay of red and green) and increased abundance on non-viable cells, as indicated by the red signal.