
Figure 1: Log10 reductions in the EN 1500 test with 15 seconds of rubbing time.
Shown are means (columns) and standard deviations (error bars) of log10 reductions in EN 1500 tests with 20 volunteers. Ethanolic (A) and propanolic hand rubs (B) were tested using 3 mL for the “responsible application” (blue) and 5 mL for the WHO 6-step method (green) for 15 seconds. The reference procedure (grey) was not modified from EN 1500 and used 2x3 mL of 60% isopropanol for 2x30 seconds.