Figure 4: HPV-unrelated carcinogenesis (left): HPV-negative primary is located at the base of tongue, FDG-PET depicts no evidence for regional metastasis (A), p16 expression is absent (C). β-Catenin (green) is located at HNSCC culture-cell surfaces, nuclei are DAPI blue-labeled (E).
HPV-related carcinogenesis (right): HPV-associated primary of the tonsil, FDG-PET depicts neck metastasis (B). p16 expression is strong (D) during fluorescence labeling nucleus (blue) and p16 protein (red) are shown. β-Catenin (green) is located to the nucleus assigning EMT phenotype (F).