gms | German Medical Science

24rd Annual Meeting of the German Retina Society

German Retina Society

17.06. - 18.06.2011, Aachen

Imaging modalities in an experimental mouse model of choroidal neovascularization

Meeting Abstract

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  • Anne Friederike Alex - Universitäts-Augenklinik Münster
  • P. Heiduschka - Universitäts-Augenklinik Münster
  • N. Eter - Universitäts-Augenklinik Münster

German Retina Society. 24th Annual Conference of the German Retina Society. Aachen, 17.-18.06.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11rg05

DOI: 10.3205/11rg05, URN: urn:nbn:de:0183-11rg055

This is the translated version of the article.
The original version can be found at: http://www.egms.de/de/meetings/rg2011/11rg05.shtml

Published: June 15, 2011

© 2011 Alex et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

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Background: The aim of the study was to evaluate new imaging modalities of the fundus in a model of choroidal neovascularization in mice which enable long term in-vivo monitoring.

Methods: Using a slit lamp adaptor, six to ten laser spots (200 mW, 0,1 second duration) were positioned in between the large retinal blood vessels in the fundus of wild-type mice, thus retinal pigment epithelium (RPE) and Bruchs membrane were ruptured. One, two and three weeks after laser, near infrared-, SD-OCT- and fluorescein angiography images were obtained. Afterwards, eyes were enucleated for histological examination.

Results: The laserspots were well defined in near infrared imaging. Degeneration of the RPE in laserspots could be well determined in SD-OCT. The near infrared imaging detected hyperreflective rimbs, which displayed enlargement over time. These rimbs showed thickend inner and outer retina in SD-OCT. Increased autofluorescence was first detectable after three weeks. On fluorescein angiography, increasing hyperfluorescent areas over time could be detected in and around the laserspots, representing leckage.

Conclusion: In-vivo monitoring of the development of choroidal neovascularization in the laser-induced mouse model is feasible. The described imaging modalities enable in-vivo montoring of new therapeutic approaches of choroidal neovascularization in mice and reduce the number of animals to be sacrified for histology.