gms | German Medical Science

22nd Annual Meeting of the German Retina Society

German Retina Society

26.06. - 27.06.2009, Berlin

Tissue-engineered biodegradable membranes for subretinal transplantation

Meeting Abstract

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  • Anna Katharina Salz - University Eye Clinic of Aachen
  • G. Thumann - University Eye Clinic of Aachen

German Retina Society. 22nd Annual Meeting of the German Retina Society. Berlin, 26.-27.06.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. DocRG2009-25

doi: 10.3205/09rg26, urn:nbn:de:0183-09rg261

This is the translated version of the article.
The original version can be found at: http://www.egms.de/de/meetings/rg2009/09rg26.shtml

Published: June 29, 2009

© 2009 Salz et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

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Objective: Replacement of degenerated RPE cells as a therapeutic modality for AMD requires that the transplanted cells reconstitute the normal subretinal anatomy. Since transplantation of cell suspensions has not resulted in functional improvement, we hypothesize that it will be necessary to transplant cells cultured on a substratum permissive for monolayer formation with correct polarity, thus allowing communication between transplanted cells and the exposed photoreceptors. The biodegradable substrate serves as a temporary Bruch's membrane prosthesis until the transplanted cells re-assemble their native Bruch's membrane. Here we compared in vitro growth characteristics of RPE and IPE cells and in vivo biocompatibility of potential biomaterials.

Methods: ARPE-19, primary bovine RPE and IPE cells cultured on ultrathin (7μm) equine collagen type I membranes, human amniotic membranes, silk membranes and polylactic acid (PLA) electrospun nanofibers were analyzed for growth, viability and morphology. Membranes were examined for surface characteristics and permeability. Biocompatibility of these biomaterials was evaluated by subconjunctival transplantation in rabbits.

Results: Pigment epithelial cells formed monolayers on all membranes, but viability was best on collagen type I followed by amniotic membranes, polylactic nanofibers and silk membranes. Subconjunctival transplantation revealed excellent biocompatibility for the collagen and amniotic membranes. Collagen membranes show most approximate the surface morphological characteristics of Bruch's membrane.

Conclusions: Regarding the investigated substrates, collagen type I membranes are best suited as a support for pigment cells intended to be transplanted subretinally, since they support cell growth, viability and do not elicit any inflammatory reaction when transplanted subconjunctivally in rabbits.