gms | German Medical Science

21st Annual Meeting of the German Retina Society and 8th Symposium of the International Society of Ocular Trauma (ISOT)

German Retina Society
International Society of Ocular Trauma

19.06. - 22.06.2008, Würzburg

Trityl dyes – clinical relevance and in-vitro analysis on the blood-retinal barrier function

Meeting Abstract

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  • Stefan Mennel - Marburg/Germany

Retinologische Gesellschaft. International Society of Ocular Trauma. 21. Jahrestagung der Retinologischen Gesellschaft gemeinsam mit dem 8. Symposium der International Society of Ocular Trauma. Würzburg, 19.-22.06.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. DocISOTRG2008V133

The electronic version of this article is the complete one and can be found online at:

Published: June 18, 2008

© 2008 Mennel.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Purpose: The use of vital dyes during vitrectomy in “chromovitrectomy” allows easier removal of less recognizable structures like epiretinal membranes (EM) or the inner limiting membrane (ILM).

Methods: To evaluate a toxic effect of PB and BBG to the RPE PB and BBG has been added to an in-vitro model of the outer blood-retinal barrier to assess dye associated barrier properties. Two concentrations of PB (2,4 mg/ml, 1,2 mg/ml) and BBG (0,25 mg/ml, 2,4 mg/ml) were investigated in a model of the fluid and air-filled eye. To determine barrier properties transepithelial resistance (TER) was measured in the follow-up of 3 days.

Results: Following application of PB barrier properties showed only mild, transient and no significant decrease of TER. BBG did not cause a breakdown of the outer blood-retinal barrier by the concentration of 0,25 mg/ml in the model of the fluid-filled eye. A concentration of 2,4 mg/ml in the model of the fluid-filled eye as well as both concentrations in the model of the air-filled showed a minor decrease after 1,5 hour which was no longer observed at the 24 hour examination. TEM did not show any dye associated ultrastrucutral alterations to the RPE cells.

Conclusion: The use of trityl dyes at the posterior eye segment seems to be save concerning damage to the RPE and its barrier function especially when the dye is used in the fluid-filled eye.