gms | German Medical Science

21st Annual Meeting of the German Retina Society and 8th Symposium of the International Society of Ocular Trauma (ISOT)

German Retina Society
International Society of Ocular Trauma

19.06. - 22.06.2008, Würzburg

Limbal stem cells transplantation in chemical and thermal burns

Meeting Abstract

  • Edward Wylegala - Katowice/Poland
  • D. Dobrowolski - Katowice/Poland
  • D. Tarnawska - Katowice/Poland

Retinologische Gesellschaft. International Society of Ocular Trauma. 21. Jahrestagung der Retinologischen Gesellschaft gemeinsam mit dem 8. Symposium der International Society of Ocular Trauma. Würzburg, 19.-22.06.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. DocISOTRG2008V058

The electronic version of this article is the complete one and can be found online at:

Published: June 18, 2008

© 2008 Wylegala et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Background: To analyse the graft survival rate and stability of the corneal surface in patients, who underwent limbal stem cells transplantation. Three surgical techniques were applied: CLAU, lrCLAL, and KLAL transplantations.

Methods: Nonrandomized consecutive comparative case series study. For the analysis we qualified: 52 patients (52 eyes), 11 women and 41 men, aged from 11 to 78 years. Mean follow–up was 28,4, range from 8 to 72 months. Three groups: CLAU, lrCLAL and KLAL contained 16, 7 and 29 eyes respectively. Graft survival rate and clinical success of stem cells transplantation in impression cytology was evaluated. Kaplan Meier survival curve was used.

Results: Comparison of the graft survival and regularity of corneal surface between allo- and autografts showed statistically significant difference. Three years graft survival rate was 87,5% in autologous transplantation group and 47,2% in allogenic transplantation group. After six years it was 81,2% and 33,3% respectively. Restoration of corneal surface was confirmed by impression cytology.

Conclusions: Autotransplantation of the limbus in ocular surface burns shows significantly better long-term outcomes.