Article
Comparative study of 12C6+ and x-ray on cell cycleprogression in the human tongue squamous cell carcinoma
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Published: | September 24, 2009 |
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The aim of this study is to compare the cell cycle alterations in the human tongue squamous cell carcinoma Tb in vitro after X-ray or carbon ion irradiation. Tb cells were irradiated with 12C6+ at dose 0, 0.5, 1, 2.0, 4.0Gy or with X-ray at dose 0, 2, 4, 6, 8Gy. Propidium iodide (PI) staining and flow cytometer (FCM) were used for cell cycle measurement. Results showed that after X-ray irradiation, the Tb cell cycle was arrested in G1 stage for 2 Gy group while 4, 6 and 8 Gy groups were arrested in G2 stage. After 12C6+ irradiation, the Tb cells were inhibited significantly in G2 M the stagnation increased gradually in a dosage and time dependent manner (P<0.01). The stagnation relieved at 24h for 0.5 Gy, while this phenomenon was not occurred for 2 and 4Gy groups. The rate of stagnation in 2Gy group was 70%, which was equal to 6Gy group by X-ray irradiation. In comparison with X-ray group, the arrested effects of Tb cells in 12C6+ irradiate group are obvious.