gms | German Medical Science

48th Meeting of the Particle Therapy Co-Operative Group

Particle Therapy Co-Operative Group (PTCOG)

28.09. - 03.10.2009, Heidelberg

Comparative study of 12C6+ and x-ray on cell cycleprogression in the human tongue squamous cell carcinoma

Meeting Abstract

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  • B. Liu - Stomatology college, Lanzhou University, Lanzhou, Gansu province, China
  • W.-W. Hou - Stomatology college, Lanzhou University, Lanzhou, Gansu province, China
  • Y.-N. Wang - Medical Physics, Institute of Modern Physics, the Chinese Academy of Sciences, Lanzhou, Gansu province, China
  • M. Wang - Stomatology college, Lanzhou University, Lanzhou, Gansu province, China
  • H. Zhang - Medical Physics, Institute of Modern Physics, the Chinese Academy of Sciences, Lanzhou, Gansu province, China

PTCOG 48. Meeting of the Particle Therapy Co-Operative Group. Heidelberg, 28.09.-03.10.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. Doc09ptcog125

DOI: 10.3205/09ptcog125, URN: urn:nbn:de:0183-09ptcog1253

Published: September 24, 2009

© 2009 Liu et al.
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Outline

Text

The aim of this study is to compare the cell cycle alterations in the human tongue squamous cell carcinoma Tb in vitro after X-ray or carbon ion irradiation. Tb cells were irradiated with 12C6+ at dose 0, 0.5, 1, 2.0, 4.0Gy or with X-ray at dose 0, 2, 4, 6, 8Gy. Propidium iodide (PI) staining and flow cytometer (FCM) were used for cell cycle measurement. Results showed that after X-ray irradiation, the Tb cell cycle was arrested in G1 stage for 2 Gy group while 4, 6 and 8 Gy groups were arrested in G2 stage. After 12C6+ irradiation, the Tb cells were inhibited significantly in G2 M the stagnation increased gradually in a dosage and time dependent manner (P<0.01). The stagnation relieved at 24h for 0.5 Gy, while this phenomenon was not occurred for 2 and 4Gy groups. The rate of stagnation in 2Gy group was 70%, which was equal to 6Gy group by X-ray irradiation. In comparison with X-ray group, the arrested effects of Tb cells in 12C6+ irradiate group are obvious.