gms | German Medical Science

54. Jahrestagung der Norddeutschen Orthopädenvereinigung e. V.

Norddeutsche Orthopädenvereinigung

16.06. bis 18.06.2005, Hamburg

Autologous bone marrow-derived cells improve muscle healing after crush injury in rats

Meeting Abstract

  • corresponding author T. Winkler - Universitätsmedizin Berlin - Charité, Zentrum für Muskuloskeletale Chirurgie, Klinik für Orthopädie, Berlin
  • G. Matziolis - Berlin
  • K. Schaser - Berlin
  • C. Perka - Berlin
  • G. Duda - Berlin

Norddeutsche Orthopädenvereinigung. 54. Jahrestagung der Norddeutschen Orthopädenvereinigung e.V.. Hamburg, 16.-18.06.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05novP18

The electronic version of this article is the complete one and can be found online at:

Published: June 13, 2005

© 2005 Winkler et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Functional deficiencies following insufficient muscle regeneration after trauma remains a serious problem in orthopedic surgery. The use of autologous pluripotent cells has been followed by promising results in healing of cartilage, bone or soft tissue defects. Goal of this study was to examine whether muscle regeneration can be improved by local application of autologous bone marrow-derived cells (BMDCs) in a rat model.

One week after standardized crush injury of the left soleus muscle autologous BMDCs vs. saline in the control group were injected into the muscle of male Sprague Dawley rats (n=16). Three weeks after cell application the contraction forces of the soleus muscles were measured bilaterally, the right muscle serving as control without trauma or therapy. Sciatic nerves were electrically stimulated in fast-twitch as well as in tetanic modus.

The control animals without cell application had a reduction of tetanic contraction force to 39±10% of the control side (p<0.001), whereas BMDC-treated animals had a reduction to 53±8% (p<0.001), the difference to the control animals being statistically significant (p=0.014). Similar results were found under fast-twitch stimulation. Control animals showed a decrease to 59±12% (p<0.001) whereas

treated animals had a reduction of contraction force to 72±13% of the control side (p<0.001), the difference to the control animals again being significant (p=0.05). In both cases autologous BMD injection lead to an increase of contraction force, 14% in tetanic and 13% in fast-twitch stimulation.

We conclude that autologous BMD injection is a suitable method to improve the functional outcome after muscle crush injury.