gms | German Medical Science

10. Kongress für Infektionskrankheiten und Tropenmedizin (KIT 2010)

Deutsche Gesellschaft für Infektiologie,
Deutsche AIDS-Gesellschaft,
Deutsche Gesellschaft für Tropenmedizin und Internationale Gesundheit,
Paul-Ehrlich-Gesellschaft für Chemotherapie

23.06. - 26.06.2010, Köln

Lack of protective immunity despite high seroprevalence of neutralizing antibodies against human metapneumovirus, Bonn, Germany

Humanes Metapneumovirus: Suszeptibilität trotz hoher Seroprävalenz neutralisierender Antikörper? Untersuchungen an einer Bonner Kohorte

Meeting Abstract

  • J. Lüsebrink - Universität Bonn, Institut für Virologie, Bonn, Germany
  • C. Wiese - Universität Bonn, Institut für Virologie, Bonn, Germany
  • A. Thiel - Universität Bonn, Kinderklinik, Bonn, Germany
  • R.L. Tillmann - Universität Bonn, Institut für Virologie, Bonn, Germany
  • V. Ditt - Universität Bonn, Institut für Virologie, Bonn, Germany
  • A. Müller - Universität Bonn, Kinderklinik, Bonn, Germany
  • O. Schildgen - Universität Bonn, Institut für Virologie, Bonn, Germany; Kliniken der Stadt Köln gGmbH, Institut für Pathologie, Köln, Germany

10. Kongress für Infektionskrankheiten und Tropenmedizin (KIT 2010). Köln, 23.-26.06.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. DocP102

DOI: 10.3205/10kit157, URN: urn:nbn:de:0183-10kit1574

Published: June 2, 2010

© 2010 Lüsebrink et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Introduction: Despite high perpetual seroprevalence-rates hMPV-reinfections occur frequently in all age groups. Our results demonstrate high neutralizing capacities of sera with minor decrease in individuals >69 years, leading to the hypothesis that reinfections occur because humoral immune responses play minor roles in clearance of HMPV-infections.

Material and methods: A total number of 2,000 patients' sera were randomly collected and screened for their neutralizing capacity, using the XTT-based neutralization-test.

Results: 96.21% of the analysed sera showed neutralizing effects. The majority of negative sera resides in the cohorts of the 0–9 year olds, which showed the least virus neutralization capacity, and the 70–79 year old patients. On the average the neutralizing effect is ~16% higher in the 29–69 year old patients compared to the group under 10 years. By trend a decrease of the neutralizing ability of the sera can be seen in patients older than 70 years.

Conclusions: The neutralization test does not identify the total amount of specific hMPV-antibodies but the ability of a patients' serum to neutralize the virus.Young children have not established a mature immune answer as they have not been in contact with as many pathogens as adults have. The elderly in contrast suffer from immunosenescence characterized by failures of the T-lymphocyte system. In concert with the results presented here this leads to the hypothesis that it is rather the hosts' T-cell response that clears the hMPV infection than the neutralizing humoral response.