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10. Kongress für Infektionskrankheiten und Tropenmedizin (KIT 2010)

Deutsche Gesellschaft für Infektiologie,
Deutsche AIDS-Gesellschaft,
Deutsche Gesellschaft für Tropenmedizin und Internationale Gesundheit,
Paul-Ehrlich-Gesellschaft für Chemotherapie

23.06. - 26.06.2010, Köln

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Prevalence of Metallo-β-lactamases (MBLs) in Clinical Isolates of Acinetobacter baumannii in Tabriz, Iran

Prävalenz von Metallo-β-Laktamasen (MBLS) in klinischen Isolaten von Acinetobacter baumannii in Tabriz, Iran

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  • A. Peymani - Ghazvin University of Medical Sciences, Department of Microbiology, Ghazvin, Iran; Tabriz University of Medical Sciences, Tabriz, Iran
  • M.R. Nahaei - Tabriz University of Medical Sciences, Tabriz, Iran
  • S. Farajnia - Tabriz University of Medical Sciences, Tabriz, Iran
  • A. Hasani - Tabriz University of Medical Sciences, Tabriz, Iran
  • A. Mirsalehian - Tehran University of Medical Sciences, Tehran, Iran
  • N. Sohrabi - Tabriz University of Medical Sciences, Tabriz, Iran
  • L. Abbasi - Zanjan University of Medical Sciences, Zanjan

10. Kongress für Infektionskrankheiten und Tropenmedizin (KIT 2010). Köln, 23.-26.06.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. DocP28

doi: 10.3205/10kit084, urn:nbn:de:0183-10kit0849

Published: June 2, 2010

© 2010 Peymani et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Objectives: Metallo-β-lactamases (MBLs) producing Acinetobacter baumannii has become a growing therapeutic concern. The aims of this study were to assess the antimicrobial susceptibility pattern of A. baumannii isolates and to determine the prevalence of MBL genes among carbapenem resistant isolates.

Methods: One hundred strains of A. baumannii were collected between March 2008 and June 2009 from different clinical samples of inpatient admitted in the largest teaching hospital in Tabriz, Iran. Antimicrobial susceptibility of all isolates was determined using the Kirby Bauer disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guideline. The isolates with reduced susceptibility to imipenem and meropenem were screened for MBL production using MBL E-test method (AB Biodisk) and were then subjected to PCR for detection of MBL genes of types bla IMP and bla VIM.

Results: Ampicilin and cefpodoxime showed the highest rates of resistance (100%) among antimicrobials tested whereas ampicillin-sulbactam and amikacin revealed relatively low resistance rates as (80%) and (83%), respectively. Among 63 carbapenem-non-susceptible isolates, 31(49.20%) were found to be MBL producer. Among 31 MBL producing isolates 19 (61.29%) carried the bla IMP gene and 9 (29.03%) carried the bla VIM gene.

Conclusion: This is the first report of bla IMP and bla VIM genes among MBL-producing A. baumannii in Iran. Considering the high frequency of MBL production among carbapenem resistant A. baumannii isolates the use of appropriate therapy and infection control measures is necessary.