gms | German Medical Science

27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Liga zur Bekämpfung des hohen Blutdrucks – Deutsche Hypertonie Gesellschaft e. V.

26. bis 29.11.2003, Bonn

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In vitro markers do not correlate with the intraarterial assessment of oxidative stress in subjects with essential hypertension

In vitro Marker korrelieren nicht mit der intraarteriellen Bestimmung von oxidativem Stress bei Patienten mit Hypertonie

Meeting Abstract (Hypertonie 2003)

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  • presenting/speaker M. Schneider - Universität Erlangen-Nürnberg (Nürnberg, D)
  • S. John - Universität Erlangen-Nürnberg (Nürnberg, D)
  • C. Delles - Universität Erlangen-Nürnberg (Nürnberg, D)
  • B. Schmidt - Universität Erlangen-Nürnberg (Nürnberg, D)
  • T. Schwarz - Universität Erlangen-Nürnberg (Nürnberg, D)
  • R. Schmieder - Universität Erlangen-Nürnberg (Nürnberg, D)

Hypertonie 2003. 27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Bonn, 26.-29.11.2003. Düsseldorf, Köln: German Medical Science; 2004. Doc03hochP88

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hoch2003/03hoch188.shtml

Published: November 11, 2004

© 2004 Schneider et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Introduction

Several previous studies have documented increased oxidative stress in the human forearm vasculature of subjects with essential hypertension (EH). Since intraarterial assessment of oxidative stress requires arterial cannulation, we sought to determine whether in vitro markers correlate with the invasive assessment and thus may provide a reliable substitute.

Methods

The effect of the acute intraarterial administration of VITC (18mg/min) on vascular function was examined in 15 hypertensive (EH) and 15 normotensive subjects (NT). Vascular function was determined as forearm blood flow (FBF) measured by plethysmography in response to the intraarterial administration of acetylcholine (ACH for EDV) and nitroprusside (NP for EIV) in doses of 12 and 48 mg/min and 32 and 128 mmol/min, respectively. As in vitro markers of oxidative stress, total antioxidative capacity of serum, oxidized LDL cholesterol concentration and hydrogen peroxide production of monocytes (by gated flow cytometry) have been determined.

Results

Subjects with EH had impaired EDV (D%FBF to ACH 48 mg/min: 319±132 vs 489±185 in NT; p=0.02) while EIV was similar to NT. Intraarterial administration of VITC restored EDV in EH (before 319±133 vs after 406±191, p=0.05). Total antioxidative capacity of serum, oxidized LDL cholesterol concentration and hydrogen peroxide production of monocytes (at baseline and after stimulation with staphylotoxin) were not different between EH and NT. Furthermore, no correlations between the intraarterial response to VITC and these three in vitro markers were found.

Discussion

VITC improves EDV in subjects with EH but not in NT documenting that oxidative stress is increased in EH. The in vitro markers studied were not elevated in EH and did not correlate with the intraarterial response to VITC. Thus, the value of these in vitro markers of oxidative stress as surrogate parameters for oxidative stress in vivo has to be questioned.