gms | German Medical Science

80th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

20.05. - 24.05.2009, Rostock

Nasal epithelial cell line stimulation with proteinase-3

Meeting Abstract

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 80th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Rostock, 20.-24.05.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. Doc09hno102

DOI: 10.3205/09hno102, URN: urn:nbn:de:0183-09hno1023

Published: July 22, 2009

© 2009 Lammers et al.
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Outline

Text

Introduction: Wegener´s Granulomatosis (WG) is a rare (incidence of 12/106 per year) autoimmune disorder which starts as granulomatous disease in the upper and lower respiratory tract, before it converts to systemic autoimmune vasculitis (generalized WG) associated with highly specific antineutrophil cytoplasmic autoantibodies against proteinase 3 (PR3-ANCA) after a variable period of time.

Oral epithelial cells could be stimulated by proteinase (PR)-3 to produce interleukin 8 (IL-8) via activation of the proteinase activated receptor (PAR)-2.

A possible stimulatory effect of PR-3 for nasal epithelial cells is unknown.

Method: The expression of PAR-2 on the immortal nasal cell line RPMI-2650 was analyzed by immunocytochemistry. Preconfluent monolayers of the cell-line were stimulated by PR-3 (10µg/ml) for 24h and 48h and the IL-8-concentration in the supernatant was quantified by ELISA. As control two unstimulated wells were analyzed.

Results: The expression of PAR-2 on RPMI-2650 could be demonstrated by immunocytochemistry. Neither after 24h nor after 48h a significant difference compared to controls could be detected.

Discussion: Cells of the immortal cell-line RPMI-2650 express the PAR-2-receptor on the outer membrane. It was not possible to stimulate a secretion of the proinflammatory cytokine IL-8 by PR-3. Further investigations of the signaling pathways (PAR-2, NF-kappa B) are planned as well as investigations of possible inhibiting effects in the described serum containing assay.


References

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Uehara A, Sugawara S, Muromato K, Takada H. Activation of Human Oral Epithelial Cells by Neutrophil Proteinase 3 Through Protease-Activated Receptor-2. J Immunol. 2002;169(8):4594-603.
2.
Rudack C, et al. RAP-2 activation regulates IL-8 and GRO-α synthesis by NF-κB, but not RANTES, IL-6, eotaxin or TARC expression in nasal epithelium. Clin Exp Allergy. 2007;37(7):1009-22.