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78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

16.05. - 20.05.2007, Munich

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In vitro effect of BDNF-producing fibroblasts on neonatal spiral ganglion cells

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  • corresponding author Athanasia Warnecke - Hannover Medical School, Hannover, Germany
  • Kirsten Wissel - Hannover Medical School, Hannover, Germany
  • Nurdanat Berkingali - Hannover Medical School, Hannover, Germany
  • Thomas Lenarz - Hannover Medical School, Hannover, Germany
  • Timo Stöver - Hannover Medical School, Hannover, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Munich, 16.-20.05.2007. Düsseldorf, Köln: German Medical Science; 2007. Doc07hno013

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2007/07hno013.shtml

Published: August 8, 2007

© 2007 Warnecke et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Neurotrophic factors, especially brain-derived neurotrophic factor (BDNF), have a protective effect on spiral ganglion cells (SGC). The aim of the present study was to demonstrate the neurotrophic effect of transfected fibroblasts expressing BDNF to induce neurite outgrowth of cultured spiral ganglion cells.

Murine NIH3T3 fibroblasts were transfected using a lentiviral vector. The resulting cell line produces BDNF and green fluorescent protein as a marker. Spiral ganglion cells were dissected from neonatal rats and were cultured after their dissociation for a period for 52 hours. Cells were cultured in serum-containig supernatant, that was derived after the cultivation of BDNF-producing fibroblasts and after the cultivation of non-transfecetd fibroblasts as control. Serum-free and serum-containing DMEM served as negative control. In parallel all groups were supplementend with BDNF and served as positiv controls. Cells cultured in medium that was supplemented with BDNF showed improved survival rates. Longest neurites and highest survival rates were achieved in SGC cultured in supernatant from BDNF-producing fibroblasts. Our results indicate, that transfecetd fibroblasts produce BDNF that has asignificant impact on surival and neurite outgrowth.