gms | German Medical Science

76th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 08.05.2005, Erfurt

Chromosome instability as detected by FISH predicts malignant outgrouwth of precursor

Meeting Abstract

  • corresponding author Johannes Jan Manni - HNO-Klinik der Universität Maastricht
  • Annemiek Haesevoets - Institut für molekulare Zellbiologie der Universität Maastricht
  • Ad Groenestein - Institut für molekulare Zellbiologie der Universität Maastricht
  • Freek Bot - Abteilung Pathologie der Universität Maastricht
  • Sandra Claessen - Institut für molekulare Zellbiologie der Universität Maastricht
  • Anton Hopman - Institut für molekulare Zellbiologie der Universität Maastricht
  • Frans Ramaekers - Institut für molekulare Zellbiologie der Universität Maastricht
  • Ernst Jan Speel - Institut für molekulare Zellbiologie der Universität Maastricht

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno657

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2005/05hno163.shtml

Published: September 22, 2005

© 2005 Manni et al.
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Outline

Text

The dilemma in managing patients with precursor lesions of the head and neck mucosa is of deciding which lesions or mucosal areas are potentially malignant and will progress to carcinoma. In a previous study on a small series of laryngeal precursor lesions we showed that an unstable chromosome content (indicated by the presence of chromosome imbalances and/or polyploidization using fluorescence in situ hybridization (FISH) for chromosome centromeres 1 and 7) strongly predicted its malignant progression [1]. To further substantiate these data for clinical application, we have now applied chromosome 1 and 7 FISH to analyze tissue sections of laryngeal precursor lesions of 103 patients, initially collected on basis of dysplastic features. Medical records were screened for patient data, and results were statistically evaluated. Apart from an increase in chromosome instability in histologically more advanced premalignant lesions, only in 6/53 (11%) lesions with a stable chromosome content the lesion progressed to a tumor (predominantly diploid tumors), whereas in 21/50 (42%) lesions with an unstable chromosome content malignant outgrouwth was observed (P=0.0009). Thus, laryngeal tumorigenesis is associated with development of chromosome instability, which can efficiently identify potentially malignant lesions. Ways to further improve the prognostic value of this approach will be discussed.

Supported by the Medical Research Foundation of the University Hospital Maastricht.


References

1.
Veltman, et al. J Clin Oncol. 2000:1644-51.