gms | German Medical Science

76th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 08.05.2005, Erfurt

Biomarker to detect genotoxic activities in the oral cavity in correlation to the oral hygiene

Meeting Abstract

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  • corresponding author Marc Bloching - Martin-Luther-University Halle-Wittenberg, Halle
  • Waldemar Reich - MLU Halle-Wittenberg, Halle
  • Tamara Grummt - Federal Environmental Agency, Bad Elster

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno460

The electronic version of this article is the complete one and can be found online at:

Published: September 22, 2005

© 2005 Bloching et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Background: Valid biomarker for recognition of high risk patients can become in future the basis of prevention-studies. Until now the influence of oral health including oral hygiene, dental status and human oral microflora on biomarker in the oral cavity is unknown. Hence, we evaluated two different biomarkers (micronucleus – assay and Ames – test for saliva testing), which are routinely used in our department to detect humans with an increased cancer risk in the upper aerodigestive tract to examine the influence of oral hygiene on the carcinogenesis in the oral cavity.

Patients and Methods: 100 healthy probands with different oral hygiene and dental status were included in the above mentioned study. The micronucleus frequency was counted up to thousand buccal cells in the exfoliative cytology. Saliva specimen were analyzed for genotoxic activity using the Ames – test in different settings (Salmonella typhimurium TA 98 and TA 100 with or without S9 – mixture) The measured values were correlated to the oral health using the SPSS software pack.

Results: Study participants with a bad oral hygiene below the average of the normal population including dental caries and periodontal status showed significantly increased genotoxic activity of saliva specimen and an increased rate of buccal micronuclei as a sign of chromosomal damage of the mucosa. Participants with composite fillings of decayed teeth showed also increased measurements of the above mentioned biomarker in correlation to patients with amalgam filled teeth.