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76th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 08.05.2005, Erfurt

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Prevelance of human papillomavirus (HPV) in cholesteatomas

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  • corresponding author Markus Fischer - Department of Oto-Rhino-Laryngology, University of Duisburg-Essen, Essen
  • Franz Peter - Department of Oto-Rhino-Laryngology, University of Duisburg-Essen, Essen
  • Teschendorf Melanie - Department of Oto-Rhino-Laryngology, University of Duisburg-Essen, Essen

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno602

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2005/05hno091.shtml

Published: September 22, 2005

© 2005 Fischer et al.
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Outline

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Introduction: Only few investigations on the prevalence of HPV-DNA in cholesteatomas have hitherto been reported. The data in literature is conflicting. Chao et al. revealed HPV-DNA in 1/32 cholesteatomas of the middle ear. Bergmann et al. detected HPV-DNA in 32% of cholesteatomas.

Material and methods: A two-step PCR was carried out to perform a broad-spectrum-assay and to increase the sensitivity. The primers were able to amplify a conserved region in the open reading frame L1.

Results: 28 cholesteatomas (18 middle ear, 10 meatal) of 28 patients (10 female/18 male) were investigated. Only one nested-PCR was slightly positive. Sequencing is still in progress and results are not available until now.

Conclusion: The detection of HPV in cholesteatomas is still no proof for their causal role in the development of cholesteatomas. As in our study even the prevalence of HPV in cholesteatomas was very low their causal relationship with the development of cholesteatomas seems to be very doubtful.