Article
New possibility of amniotic membrane preparation with the water jet
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Authors
Published: | September 22, 2004 |
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Outline
Text
Objective
Amniotic membrane transplantation is used in the ophthalmology for ocular surface reconstruction. The mechanism of the regeneration of the corneal surface after Amniotic membrane grafting is not well established. This study shows a new technology to remove the epithelial cells from amniotic membrane with the water jet.
Methods
40 Amniotic membrane obtained from caesarean deliveries were prepared under sterile condition in the eye banc of the eye clinic of the Ernst-Moritz-Arndt- university of greifswald. The remove of epithelial cells from the amniotic membrane with the water jet under various pressures, systems, using of aqua dest.. The investigation was performed by light and scanning electron microscopy. One group (10) without polishing and another group (10) after polishing with water jet. We compared the results.
Results
The results show that the epithelial cells of the amniotic membrane were considerably reduced after water jet procedure.
Conclusions
The transplantation of amniotic membrane without epithelial cells appears to be a promising in the surgical treatment for reconstructing the ocular surface.