gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Diagnostic approach in primary intraocular lymphoma by transretinal biopsy

Meeting Abstract

  • corresponding author O. Vij - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen
  • R. Bölöni - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen
  • S. Coupland - Institut für Pathologie, Universitätsklinikum Benjamin Franklin, FU Berlin, Berlin
  • N. Bornfeld - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogFR.11.04

The electronic version of this article is the complete one and can be found online at:

Published: September 22, 2004

© 2004 Vij et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.




Due to frequently variable clinical course and a broad spectrum of differential diagnosis in primary intraocular lymphoma a cytological examination is often indicated. Biopsy methods should guarantee a minimum of surgical risks with cytological irreproachable results. Vitreous biopsy in PIOL is often limited by inconclusive cytopathologic diagnosis caused by inadequate sampling. 5 cases are presented, in which previous vitreous biopsy result was not evident. After transretinal biopsy of elevated subretinal lesions cythopathologic examination verified PIOL.


5 female patients with a mean age of 66 years, showed nondiagnostic vitreous biopsy in subretinal lesions suspicious for PIOL. Biopsy with the ocutome was obtained by core vitrectomy and retinotomy. Standardized cytopathologic analysis was performed without complications. 1 patient underwent transretinal biopsy 2 years after inconclusive vitreous biopsy in the contralateral eye.


In 4 patients the diagnosis of B-cell, in 1 case of T-cell non-Hodgkin type lymphoma was established. CNS manifestation was found in 2 patients, in one case the staging is not finished yet. Tumour regression was obtained by chemotherapy in 3 patients and combined radiation with chemotherapy in 1 patient. During an average follow-up of 13.5 months, one tumour recurrence occured in the same eye.


In suspicious cases for PIOL and inconclusive cytopathologic diagnosis due to inadequate vitreous biopsy a transretinal approach should be considered in elevated subretinal lesions. This method is safe and well manageable and seems to be superior to fine needle aspiration.