gms | German Medical Science

German Congress of Orthopedic and Trauma Surgery (DKOU 2017)

24.10. - 27.10.2017, Berlin

Bone sialoprotein-coated 3D-printed hydroxyapatite scaffolds stimulate bone healing in a rat model

Meeting Abstract

  • presenting/speaker Andreas Baranowski - Unimedizin Main, Zentrum für Orthopädie und Unfallchirurgie, Mainz, Germany
  • Anja Klein - Unimedizin Main, Zentrum für Orthopädie und Unfallchirurgie, Mainz, Germany
  • Ulrike Ritz - Unimedizin Main, Zentrum für Orthopädie und Unfallchirurgie, Mainz, Germany
  • Alexander Hofmann - Unimedizin Main, Zentrum für Orthopädie und Unfallchirurgie, Mainz, Germany
  • Pol M. Rommens - Unimedizin Main, Zentrum für Orthopädie und Unfallchirurgie, Mainz, Germany
  • Hermann Götz - Institute of Applied Structure- and Microanalysis, Mainz, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2017). Berlin, 24.-27.10.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. DocPO25-437

doi: 10.3205/17dkou813, urn:nbn:de:0183-17dkou8132

Published: October 23, 2017

© 2017 Baranowski et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Fragestellung: It is a difficult road from idea to implementation of a biomaterial that is able to replace conventional bone grafts in the treatment of large-scale bone defects. 3-dimensional (3D) plotting introduced solutions to some contradictory requirements for bone substitutes. 3D-printed scaffolds made of calcium deficient hydroxyapatite (CDHA) provide degradability and combine stability and composition with a design of interconnected macropores. Thus cell infiltration, neovascularization and exchange of products of metabolism are possible and allow an ingrowth of bone. In earlier in vitro studies we found an enhanced proliferation of osteoblasts on Bone sialoprotein (BSP)-coated bone substitutes and an increased gene expression of bone marker genes (SPARC, SP7, Runx2, OPN). In this project we want to show the in vivo effect of BSP-coated 3D printed CDHA-scaffolds on bone healing in a murine calvarial defect model.

Methodik: A 2.5 mm diameter critical size defect was created in mice calvaria. 3D-printed CDHA scaffolds (Innotere, Radebeul) with interconnecting pores were BSP-coated via physisorption and implanted into the defects. As a positive control scaffolds coated with Bone morphogenetic protein (BMP) 7 were implanted. Another group contained uncoated scaffolds. Untreated calvarial defects served as negative control. The mice were euthanized and decapitated 8 weeks after the implantation. Micro-CT examinations and histological analyses followed.

Ergebnisse und Schlussfolgerung: BSP-coating of 3D-plotted CDHA scaffolds resulted in thicker bone at the implant-bone interface compared to CDHA scaffolds alone. In BV/TV (Bone Volume/Total Volume) measurements, BSP-coated CDHA tended to result in higher bone growth in comparison to the other groups. Histological stainings reflected these findings. Conclusion: BSP-coated CDHA scaffolds stimulated bone ingrowth.