gms | German Medical Science

Deutscher Kongress für Orthopädie und Unfallchirurgie, 75. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 97. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 52. Tagung des Berufsverbandes der Fachärzte für Orthopädie und Unfallchirurgie

25. - 28.10.2011, Berlin

Exercise enhances angiogenesis during bone defect healing in mice

Meeting Abstract

  • J. Holstein - Universitätsklinikum des Saarlandes, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Homburg, Germany
  • S. Becker - Universitätsklinikum des Saarlandes, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Homburg, Germany
  • P. Garcia - Universitätsklinikum des Saarlandes, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Homburg, Germany
  • T. Histing - Universitätsklinikum des Saarlandes, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Homburg, Germany
  • T. Pohlemann - Universitätsklinikum des Saarlandes, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Homburg, Germany
  • M. Menger - Universitätsklinikum des Saarlandes, Institut für klinisch-experimentelle Chirurgie, Homburg, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie. 75. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 97. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 52. Tagung des Berufsverbandes der Fachärzte für Orthopädie. Berlin, 25.-28.10.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. DocGR13-77

DOI: 10.3205/11dkou462, URN: urn:nbn:de:0183-11dkou4624

Published: October 18, 2011

© 2011 Holstein et al.
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Outline

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Questionnaire: The aim of the present study was to investigate the effect of physical exercise on angiogenesis during bone defect healing in mice.

Methods: We evaluated angiogenesis during bone repair in a cranial bone window model by intravital fluorescence microscopy (IVM) at days 0, 3, 6, 9, 12, 15, 18, and 21. In addition, we performed histomorphometric analyses at days 3, 6, 9, and 15 to characterize the type and time course of bone repair. IVM analyses were conducted in mice, which were housed in cages supplied with running wheels (exercise group; n=7) and in mice, which were housed in standard cages without running wheels (controls; n=7). The running distance of each animal was calculated by a digital rev counter. Results of the IVM analyses were compared between the two groups by U-Test (Mann-Whitney). In the exercise group, we additionally performed correlation analyses (Pearson) between results of the IVM and the running distance.

Results and Conclusions: Mice of the exercise group run a mean distance of 3.6km/d. IVM showed an accelerated decrease of bone defect area in the exercise group when compared to the control group. This was associated with a significantly higher blood vessel diameter in animals undergoing exercise at days 9 and 12 and a significant positive correlation between running distance and blood vessel density at day 9 (r=0.74). Histomorphometry showed a typical pattern of intramembranous bone repair with osseous bridging of the defect at day 9. The newly woven bone was covered by a neo-periosteum containing those blood vessels, which were visible by IVM. We conclude that exercise accelerates bone defect healing and stimulates angiogenesis during bore repair.