gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

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Intravesically applied immunostimulatory DNA (CpG-ODN) reduces tumor load in an orthotopic murine transitional cell carcinoma (TCC) model (MB-49/ C57-Bl6)

Meeting Abstract

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  • corresponding author presenting/speaker Peter Olbert - Klinik für Urologie und Kinderurologie, Philipps-Universität Marburg, Deutschland
  • Andres Jan Schrader - Klinik für Urologie und Kinderurologie, Philipps-Universität Marburg
  • Peter Barth - Institut für Pathologie, Philipps-Universität Marburg
  • Rainer Hofmann - Klinik für Urologie und Kinderurologie, Philipps-Universität Marburg
  • Axel Hegele - Klinik für Urologie und Kinderurologie, Philipps-Universität Marburg

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPE322

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dkk2006/06dkk432.shtml

Published: March 20, 2006

© 2006 Olbert et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objectives: BCG is established in the prophylaxis of superficial bladder cancer. It induces an unspecific, Th-1-biased local immune response. ODN containing a central unmethylated CpG motif are able to mimick the immunostimulatory activity of bacterial DNA. Purpose of the present study was to evaluate the antineoplastic properties of intravesically administered CpG-ODN in an orthotopic murine bladder cancer model.

Methods: MB-49 tumor cell suspension was instilled transurethrally in female C57/BL6 mice. Mice were divided in 3 groups of 12 animals. 4 in each group received either stimulative CpG ODN, non-stimulative GpC ODN or PBS intravesically: group I on day 3, group II on day 5, group III on day 7. After sacrifice 7 days after treatment bladders were removed and histological examinations were performed.

Results: Single instillation of CpG ODN revealed antineoplastic effects in every group reflected by significantly lower bladder weight (p>0.05) compared to GpC ODN and PBS treated mice. Histological examination showed extensive infiltration of macrophages and lymphocytes in CpG ODN treated mice, whereas PBS and GpC ODN treated mice showed solid tumor growth without signs of immunological response.

Conclusions: Intravesically applied immunostimulative DNA demonstrated antitumoral activity in an orthotopic murine bladder cancer model. A single instillation seems to be sufficient to reduce tumor load.