gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

Article

Send article

Disturbance of apoptosis regulators during tumour progression in renal cell carcinomas

Meeting Abstract

Search Medline for

  • corresponding author presenting/speaker Uwe Ramp - Institut für Pathologie, Universitätsklinikum Düsseldorf, Deutschland
  • Sebastian Heikaus - Institut für Pathologie, Universitätsklinikum Düsseldorf
  • Yong Yan - Institut für Pathologie, Universitätsklinikum Düsseldorf
  • Csaba Mahotka - Institut für Pathologie, Universitätsklinikum Düsseldorf
  • Helmut Erich Gabbert - Institut für Pathologie, Universitätsklinikum Düsseldorf

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPO306

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dkk2006/06dkk416.shtml

Published: March 20, 2006

© 2006 Ramp et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Aims: The X-linked inhibitor of apoptosis (XIAP) is one of the most potent caspase inhibitors of the inhibitor of apoptosis (IAP)-family members. Only recently, an antagonist of XIAP has been identified, named Smac/DIABLO. Since a dysbalance of pro- and antiapoptotic regulators are thought to play a major role in tumor progression, the aim of the present study was to explore the relevance of antiapoptotic XIAP and proapoptotic Smac/DIABLO for tumour progression in renal cell carcinomas (RCCs).

Methods: XIAP and Smac/DIABLO mRNA and protein expression was analysed in primary tumour tissue from 66 RCCs of all major histological types by quantitative real-time PCR, Western blot and ELISA.

Results: 1. XIAP and Smac/DIABLO mRNA expression was found in all RCCs. 2. The relative XIAP mRNA expression levels significantly increased from early (pT1) to advanced (pT3) tumour stages (p=0.0002) and also with tumour dedifferentiation (p=0.04). 3. Western blot analysis confirmed the tumour stage-dependent increase of XIAP expression on the protein level. 4. In contrast, mRNA and protein expression levels of Smac/DIABLO did not significantly change between early and advanced tumour stages or between low and high tumour grades. 5. The mRNA expression ratio between XIAP and Smac/DIABLO markedly increased during progression from early (pT1) to advanced (pT3) tumour stages.

Conclusions:.The delicate balance between antiapoptotic XIAP and proapoptotic Smac/DIABLO expression is gradually disturbed during progression of RCCs, resulting in a relative increase of XIAP over Smac/DIABLO, thereby probably contributing to the marked apoptosis resistance of RCC.