gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

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Experimental Models of monocytic infiltration into gliomas

Meeting Abstract

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  • corresponding author presenting/speaker Herwig Strik - Dep. of Neurology, Göttingen, Deutschland
  • Petra Hülper - Dep. of Pediatrics 1, Göttingen
  • Bernhard Hemmerlein - Dep. of Pathology, Göttingen
  • Bernhard Erdlenbruch - Dep. of Pediatrics 1, Göttingen
  • Jessica Meier - Dep. of Neurology, Göttingen
  • Alexandra Kowalewski - Dep. of Neurology, Göttingen
  • Ralf Gold - Institute of MS Research, Göttingen
  • Mathias Bähr - Dep. of Neurology, Göttingen

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPO277

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dkk2006/06dkk387.shtml

Published: March 20, 2006

© 2006 Strik et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objective: human malignant gliomas are extensively infiltrated by mononuclear cells. Cellular anti-tumour immune response, however, is not observed. To further investigate this phenomenon, we established models of monocytic invasion into glioma cell aggregates in vitro and in vivo.

In the in vitro model, peripheral blood monocytes infiltrate regularly into glioma cell spheroids, but also into SY5Y neuroblastoma cell spheroids. Coexpression of both MRP8 and MAC387 (MRP14) and expression of 25F9 but absence of 27E10 in the glioma cell spheroids point towards a chronic inflammatory phenotype of the infiltrating monocytes.

In vivo, labelled peritoneal macrophages infiltrate into the parenchyma of syngeneic cerebral rat gliomas both after intraarterial and intravenous injection.

The experimental models presented here allow to analyse interactions between monocytes and tumour cells both in vitro and in vivo and may help to understand mechanisms of monocytic inactivation by glioma cells. Moreover, the specific infiltration from the peripheral blood opens the possibility to use peripheral blood monocytes as vehicles to launch therapeutic agents specifically into cerebral gliomas.