gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

Coculture of human breast cancer cells with human primary osteoblasts (hOB) – Influence on migration and invasion

Meeting Abstract

  • corresponding author presenting/speaker Carsten Gründker - Universitäts-Frauenklinik, Göttingen, Deutschland
  • Julia von Alten - Universitäts-Frauenklinik, Göttingen
  • Hiltrud Schulz - Universitäts-Frauenklinik, Göttingen
  • Günter Emons - Universitäts-Frauenklinik, Göttingen

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocOP060

The electronic version of this article is the complete one and can be found online at:

Published: March 20, 2006

© 2006 Gründker et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Bone, besides lung and liver, is one of the most preferential metastatic target sites for breast cancers. Although the precise molecular mechanisms underlying this preference need to be elucidated, it appears that bone microenvironments possess unique biological features that enable circulating cancer cells to home, survive and proliferate, and destroy bone. The crosstalk between metastatic breast cancer cells and bone is critical to the development and progression of bone metastases. Disruption of this interaction will allow us to design mechanism-based effective and specific therapeutic interventions for bone metastases. We have established a coculture system of different breast cancer cell lines stable transfected with red fluorescence and human primary osteoblasts (hOB) or MG63 human osteosarcoma cells to analyze tumor cell migration and invasion to bone. We could show that breast cancer cell invasion was increased when cocultured with hOB or MG63. Using this model we will examine the influence of various pharmaceuticals on migration and invasion of the tumor cells on cellular as well as molecular level.