gms | German Medical Science

45. Kongress der Deutschen Gesellschaft für Rheumatologie, 31. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie, 27. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie

06.09. - 09.09.2017, Stuttgart

Differential influence of synovial fibroblasts from osteoarthritis vs. rheumatoid arthritis patients on B cell survival and immunoglobulin production

Meeting Abstract

  • Dennis Bleck - Universitätsklinikum Düsseldorf, Poliklinik, Funktionsbereich & Hiller Forschungszentrum für Rheumatologie, Düsseldorf
  • Torsten Lowin - Universitätsklinikum Düsseldorf, Funktionsbereich & Hiller Forschungszentrum für Rheumatologie, Düsseldorf
  • Matthias Schneider - Poliklinik, Funktionsbereich & Hiller Forschungszentrum für Rheumatologie, UKD, Heinrich-Heine-Universität Düsseldorf, Düsseldorf
  • Georg Pongratz - Universitätsklinikum Düsseldorf, Poliklinik, Funktionsbereich & Hiller Forschungszentrum für Rheumatologie, Düsseldorf

Deutsche Gesellschaft für Rheumatologie. Deutsche Gesellschaft für Orthopädische Rheumatologie. Gesellschaft für Kinder- und Jugendrheumatologie. 45. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh), 31. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh), 27. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR). Stuttgart, 06.-09.09.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. DocER.05

doi: 10.3205/17dgrh080, urn:nbn:de:0183-17dgrh0802

Published: September 4, 2017

© 2017 Bleck et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Background: Rheumatoid Arthritis (RA) is a disease characterized by chronic inflammation of the synovial tissue in joints. It is driven by highly activated B cells. Synovial cells, primarily synovial Fibroblasts (SF) play an important role in activating these B cells. In order to investigate the interactions responsible for B cell activation, a co-culture model was established with RASF and SF from Osteoarthritis (OA) patients.

Methods: IgD+ B cells were isolated from human peripheral blood by MACS and co-cultured w/wo SFs in medium only, in the presence of IFN-gamma or CpG, respectively. B cell subpopulations, survival and proliferation were determined by FACS. IgG and IgM were measured by ELISA.

Results: B cell survival is increased in co-culture after 6 days without additional stimulation (RASF: +20.6% +/-1.2%; OASF: +14.5 % +/- 5.5 %; p=0.02). Proliferation of B cells is already increased after 3 hours of co-culture without additional stimulation (RASF: +13.4 % +/- 2.1 %; OASF: +13.0 % +/- 1.1 %; ns). Addition of CpG leads to an increase of CD38+ B cells without significant differences between RASF and OASF (RASF: 21.3 % +/- 8.6 %; OASF: 16.9 % +/- 6.5 %; ns). After 6 days, an increase in IgM was detected following CpG activation, which was pronounced in the presence of RASF (RASF: from: 6.7 ng/mL +/- 0.9 ng/mL to 517.5 ng/mL +/- 228.4 ng/mL; OASF: from: 5.9 ng/mL +/- 0.3 ng/mL to 189.6 ng/ml +/-204.4 ng/ml; p=0.05). In contrast IgG, induced in the presence of IFN-gamma showed a trend towards an increase OASF co-culture after 12 days (RASF: from: 0.8 ng/mL +/-0.1 ng/mL to 37.8 ng/mL +/-18.5 ng/mL; OASF: from: 0.7 ng/mL +/-0.2 ng/mL to 51.9 ng/mL+/-10.8 ng/mL; p=0.10).

Conclusion: RASF show increased capacity to augment survival of B cells as compared to OASF. In the presence of CpG, B cells produce IgM, with higher levels in the presence of RASF. In contrast, IgG is induced in the presence of IFN-gamma with a trend to increased levels in the presence of OASF. In conclusion, RASF and OASF show different effects on B cells in terms of survival and induction of immunoglobulins.