Article
A fibrin spray system for the delivery of ASCs to diabetic wounds
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Published: | August 16, 2017 |
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Introduction: Adipose derived stem cells (ASCs) are a promising regenerative therapy for chronic wounds. A problem of their therapeutic application is how to apply them to and immobilize them in the wound area. Various scaffolds have been developed but the optimal cell delivery system is not yet determined. Here we evaluated the effectiveness of a fibrin spray system for the delivery of ASCs to a immunocompromised murine diabetic wound model.
Methods: ASCs were harvested from five human adult patients undergoing elective liposuction. After collagenase digestion ASCs were isolated by flow cytometry before culture. ASCs were applied to splinted excisional wounds in NSG mice by means of the Tisseel fibrin spray system or via drip on technique. ASC engraftment and survival was evaluated via IVIS imaging. Wound healing kinetics were analyzed and histology was carried out upon wound closure.
Results: In the fibrin sealent group ASCs remained viable significantly longer after application resulting in significantly accelerated wound closure. Potentially explaining the improved healing we observed a significantly increased blood vessel count on CD31 staining in the wounds of this group.
Conclusions: Human adipose tissue is a rich source of autologous stem cells, which are readily available for regenerative applications such as wound healing. Our results indicate that ASCs can be used as an effective cell therapeutic when applied and immobilized via a clinically approved fibrin sealant spray system.