gms | German Medical Science

48. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 55. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie (ÖGPÄRC), 22. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC)

14.09. - 16.09.2017, Graz, Österreich

A comparison of the regenerative gene expression in ASCs derived from UAL, SAL and abdominoplasty samples from 5 individual patients

Meeting Abstract

  • presenting/speaker Matthias M. Aitzetmüller - Klinikum rechts der Isar, Abteilung für experimentelle Plastische Chirurgie, München, Deutschland
  • Ursula Hopfner - Klinikum rechts der Isar, Abteilung für experimentelle Plastische Chirurgie, München, Deutschland
  • Manuela Kirsch - Klinikum rechts der Isar, Abteilung für experimentelle Plastische Chirurgie, München, Deutschland
  • Hans-Günther Machens - Klinikum rechts der Isar, Abteilung für experimentelle Plastische Chirurgie, München, Deutschland
  • Dominik Duscher - Klinikum rechts der Isar, Abteilung für experimentelle Plastische Chirurgie, München, Deutschland

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Österreichische Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 48. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 55. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie, 22. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Graz, Österreich, 14.-16.09.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. Doc081

doi: 10.3205/17dgpraec081, urn:nbn:de:0183-17dgpraec0814

Published: August 16, 2017

© 2017 Aitzetmüller et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Background: Due to their regenerative potential, Mesenchymal stem cells are already in clinical use in several medical specialties. In plastic surgery, mainly the adipose-derived stromal cells (ASCs) are in the focus of recent research to remedy defects across the whole body and to reverse ischemic tissue states. The ability of the regenerative cytokine release is the key mechanism in the therapeutic use of ASCs. Through the intensive research effort of the last decades in the area of liposuction for aesthetic or reconstructive use, various methods and devices have been developed. However which method derives the most potent cells for regenerative applications is still disputed. Here we assess ASCs from Ultrasound assisted (UAL) and suction assisted (SAL) lipoaspirat as well as ASCs from excised fat for their ability to release key cytokines for tissue regeneration.

Material and methods: UAL and SAL lipoaspirate was collected from 5 patients and compared with excisional fat from abdominoplasty samples from the same patients. ASCs were characterized by their surface marker profile CD45- / CD31- / CD34 + by means of FACS and the expression of differentiation cytokines (HGF, FGF-2, MCP-1, SDF-1, VEGF) was compared.

Results: There was no significant difference between the release of HGF, FGF-2, SDF-1 and VEGF between the individual samples. However, we observed an increased expression of MCP-1 in the UAL sample.

Conclusio: Utilizing an elegant study design with the patients serving as their own controls we were able to show that UAL and SAL both derive ASCs comparable to cells from excised fat. This demonstrates these methods have potential to harvest ASCs for autologous fat transfer and regenerative medicine.