Figure 2: Bromodeoxyuridine (BrdU) proliferation assay
The effects of the indicated compounds on proliferation in A-204 (a), SK-LMS-1(b) and VA-ES-BJ cells (c) were measured by BrdU cell proliferation assay. The assay was performed following 6 hours of incubation with sodiumpyruvate (control), 250 µmol/l TRD, 250ng/ml TRAIL or with a combination of TRD and TRAIL. Data are presented as the mean±SD of 8 measurements. ANOVA was used to demonstrate statistical significance between the different categories with a Tukey’s multiple comparison post test. Significantly different at ***p=0.001, **p=0.005, *p< 0.05; ns, p>0.05). The indicators of significance refer to the difference between the treatment groups and the control series.