gms | German Medical Science

65th Annual Meeting of the German Society of Neurosurgery (DGNC)

German Society of Neurosurgery (DGNC)

11 - 14 May 2014, Dresden

Radiological evolution of contusional changes in murine model of moderate closed head injury

Meeting Abstract

  • Jacek Szczygielski - Klinik für Neurochirurgie, Universitätskliniken des Saarlandes, Homburg/Saar
  • Angelika E. Mautes - Klinik für Neurochirurgie, Universitätskliniken des Saarlandes, Homburg/Saar
  • Andreas Müller - Klinik für Radiologie, Universitätskliniken des Saarlandes, Homburg/Saar
  • Vanessa Hubertus - Klinik für Neurochirurgie, Universitätskliniken des Saarlandes, Homburg/Saar
  • Eduard Kruchten - Klinik für Neurochirurgie, Universitätskliniken des Saarlandes, Homburg/Saar
  • Karsten Schwerdtfeger - Klinik für Neurochirurgie, Universitätskliniken des Saarlandes, Homburg/Saar
  • Joachim Oertel - Klinik für Neurochirurgie, Universitätskliniken des Saarlandes, Homburg/Saar

Deutsche Gesellschaft für Neurochirurgie. 65. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC). Dresden, 11.-14.05.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. DocP 111

doi: 10.3205/14dgnc507, urn:nbn:de:0183-14dgnc5078

Published: May 13, 2014

© 2014 Szczygielski et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Objective: Magnetic resonance imaging (MRI) became a useful diagnostic tool for analysis of parenchymal changes in traumatic brain injury. The goal of our current experiment series was to assess the prolonged course of contusion in mouse model of closed head injury.

Method: Male CD-1 mice (n=7) were subjected to moderate-to-severe brain trauma according to the well-established closed head injury model. The radiological sequealae were assessed on the time points: 24h, 72h, 7 days, 14 days and 28 days after insult by magnetic resonance imaging using a 9.4 Tesla scanner.

Results: Using T2, TRARE and ADC-sequences, the focal contusional changes with early secondary haemorrhagic transformation (24 and 72h scans) were presented. The morphology of edema could be described as early posttraumatic swelling (24h and 72h scans) as well as secondary edema (7d and later) related to gradual resorption of haemorrhagic changes. The profound disturbance of water diffusion in traumatized hemisphere was displayed throughout the entire follow-up (24h up to 28 days).

Conclusions: The sequence and time course of posttraumatic contusional changes in our murine model closely resembles those seen in clinical settings of human brain trauma. Therefore, the presented model of brain trauma may be used for long-term analysis of potential treatment strategies on the level of animal experiment.