Article
Radiological evolution of contusional changes in murine model of moderate closed head injury
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Published: | May 13, 2014 |
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Outline
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Objective: Magnetic resonance imaging (MRI) became a useful diagnostic tool for analysis of parenchymal changes in traumatic brain injury. The goal of our current experiment series was to assess the prolonged course of contusion in mouse model of closed head injury.
Method: Male CD-1 mice (n=7) were subjected to moderate-to-severe brain trauma according to the well-established closed head injury model. The radiological sequealae were assessed on the time points: 24h, 72h, 7 days, 14 days and 28 days after insult by magnetic resonance imaging using a 9.4 Tesla scanner.
Results: Using T2, TRARE and ADC-sequences, the focal contusional changes with early secondary haemorrhagic transformation (24 and 72h scans) were presented. The morphology of edema could be described as early posttraumatic swelling (24h and 72h scans) as well as secondary edema (7d and later) related to gradual resorption of haemorrhagic changes. The profound disturbance of water diffusion in traumatized hemisphere was displayed throughout the entire follow-up (24h up to 28 days).
Conclusions: The sequence and time course of posttraumatic contusional changes in our murine model closely resembles those seen in clinical settings of human brain trauma. Therefore, the presented model of brain trauma may be used for long-term analysis of potential treatment strategies on the level of animal experiment.