gms | German Medical Science

63rd Annual Meeting of the German Society of Neurosurgery (DGNC)
Joint Meeting with the Japanese Neurosurgical Society (JNS)

German Society of Neurosurgery (DGNC)

13 - 16 June 2012, Leipzig

Matrix metalloproteinase (MMP)-9 expression in glioma infiltrating glia

Meeting Abstract

  • D.S. Markovic - Helios Klinikum Berlin-Buch, Neurochirurgische Klinik, Berlin; Max-Delbrück-Zentrum für Molekulare Medizin, Zelluläre Neurowissenschaften, Berlin
  • M.C. Ku - Max-Delbrück-Zentrum für Molekulare Medizin, Zelluläre Neurowissenschaften, Berlin
  • H. Kettenmann - Max-Delbrück-Zentrum für Molekulare Medizin, Zelluläre Neurowissenschaften, Berlin
  • J.C.W. Kiwit - Helios Klinikum Berlin-Buch, Neurochirurgische Klinik, Berlin

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS). Leipzig, 13.-16.06.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. DocP 091

DOI: 10.3205/12dgnc478, URN: urn:nbn:de:0183-12dgnc4789

Published: June 4, 2012

© 2012 Markovic et al.
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Outline

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Objective: Analysis of MMP-9 expression levels in glioma-infiltrating glia and examination of cross-talk between glioma cells and glial cells in the context of MMP-9 regulation.

Methods: We determined MMP-9 regulation by PCR, westernblotting and gelatinase zymography in a mouse microglia and astrocytes cell culture and in mouse glioma cell line GL261. We stimulated the cell cultures with the GL261 conditioned medium (GCM) and with the microglia conditioned medium (MCM). Additionally we used the mouse in vivo glioma model where we implanted GL261 into mouse basal ganglia. Afterwards we used immunohistochemistry and confocal microscopy to characterize the colabeling of the glial cells with MMP-9 in and around gliomas.

Results: The stimulation of primary astrocytes with GCM induced MMP-9 expression. 3h and 6h after culturing of primary astrocytes in GCM we observed a strong increase of MMP-9 expression. An increase of MMP-9 expression was also observed in primary microglial cells after GCM stimulation, but to a lesser extent. GL261 express MMP-9 only after stimulation with MCM. In situ, we observed the overexpression of MMP-9 in astrocytes and microglia surrounding glioma cells.

Conclusions: For the first time we identify the astrocytes and microglia as major suppliers of MMP-9 in gliomas. The astrocytes and microglia express increased MMP-9 after stimulation with GCM.