gms | German Medical Science

63rd Annual Meeting of the German Society of Neurosurgery (DGNC)
Joint Meeting with the Japanese Neurosurgical Society (JNS)

German Society of Neurosurgery (DGNC)

13 - 16 June 2012, Leipzig

Detection of fluorescent porphyrins after exposure of pediatric brain tumors to 5-aminolevulinic acid

Meeting Abstract

  • M. Schwake - Klinik für Neurochirurgie, Westfälische Wilhelms Universität, Münster, Deutschland
  • D. Günes - Klinik für Neurochirurgie, Westfälische Wilhelms Universität, Münster, Deutschland
  • M. Köchling - Klinik für Neurochirurgie, Westfälische Wilhelms Universität, Münster, Deutschland
  • A. Brentrup - Klinik für Neurochirurgie, Westfälische Wilhelms Universität, Münster, Deutschland
  • J. Schroeteler - Klinik für Neurochirurgie, Westfälische Wilhelms Universität, Münster, Deutschland
  • M. Frühwald - Klinik für Kinder und Jugendliche, Klinikum Augsburg, Deutschland
  • W. Stummer - Klinik für Neurochirurgie, Westfälische Wilhelms Universität, Münster, Deutschland
  • C. Ewelt - Klinik für Neurochirurgie, Westfälische Wilhelms Universität, Münster, Deutschland

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS). Leipzig, 13.-16.06.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. DocSA.02.03

DOI: 10.3205/12dgnc313, URN: urn:nbn:de:0183-12dgnc3132

Published: June 4, 2012

© 2012 Schwake et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

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Objective: Fluorescence-guided surgery with 5-aminolevulinic acid (5-ALA) makes more complete resections of contrast-enhancing tumors in adults possible. 5-ALA elicits synthesis and accumulation of fluorescent porphyrins in various epithelia and cancerous tissues, which can be visualized using a modified neurosurgical microscope with blue light. Although this technique is well established in adults, it has not been investigated systematically in children even though more complete resections of many pediatric brain tumors are assumed to improve the prognosis. We therefore investigated the feasibility of 5-ALA guided surgery in typical pediatric brain tumors in vitro.

Methods: Cell cultures of medulloblastoma [DAOY and UW228], cPNET [PFSK] rhabdoid tumor [BT1] and ependymoma [RES196] were incubated with 5-ALA for 1, 3, 6, 9, 12 and 24 hours, and the fluorescence intensities of porphyrins were determined using a fluorescence-activated cell sorter (FACS). In addition, we incubated the same cell lines with 5-ALA for only one hour and determined the fluorescence intensity after 1, 3, 6, 9, 12 and 24 hours in order to measure the kinetics of accumulation. C6 and U87-glioma cell lines served as positive controls.

Results: We found strong fluorescence signal in all pediatric brain tumor lines compared to control cells (p<0.05), similar to the signal found in C6 and U87-glioma cells.

Conclusions: We conclude that the exposure of typical pediatric cell tumors to 5-ALA induces accumulation of fluorescent porphyrins in these cells. This indicates that fluorescence-guided surgery of these tumors is feasible. FACS appears to be a reliable method for detecting porphyrin fluorescence in cell cultures.