gms | German Medical Science

63rd Annual Meeting of the German Society of Neurosurgery (DGNC)
Joint Meeting with the Japanese Neurosurgical Society (JNS)

German Society of Neurosurgery (DGNC)

13 - 16 June 2012, Leipzig

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Influence of novel AQP1 promoter haplotypes on AQP1 expression, tumor edema as well as two-years survival in patients suffering from glioblastoma multiforme

Meeting Abstract

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  • N.E. Hindy - Abteilung für Neurochirurgie, Universitätsklinikum Essen
  • M. Adamzik - Abteilung für Anästhesie und Intensivmedizin, Universitätsklinikum Essen
  • N. Lambertz - Abteilung für Neurochirurgie, Universitätsklinikum Essen
  • Y. Zhu - Abteilung für Neurochirurgie, Universitätsklinikum Essen
  • U. Sure - Abteilung für Neurochirurgie, Universitätsklinikum Essen
  • I.E. Sandalcioglu - Abteilung für Neurochirurgie, Universitätsklinikum Essen

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS). Leipzig, 13.-16.06.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. DocFR.01.10

doi: 10.3205/12dgnc174, urn:nbn:de:0183-12dgnc1746

Published: June 4, 2012

© 2012 Hindy et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective: Water channel proteins (AQP) are overexpressed in brain tumors, mediating cell proliferation and angiogenesis. We investigated the influence of two AQP1 promoter single nucleotide polymorphisms (-783C > G)/(-781C > T) defining novel AQP1 haplotypes on gene expression and their influence on peritumoral edema as well as two-years survival of patients suffering from glioblastoma multiforme.

Methods: Reporter activity, transcription factor binding and gene expression, dependent on (-783C > G)/(-781C > T) haplotypes was conducted in HELA cells and blood tissue. After genotyping of 151 patients suffering from GBM, comparison to healthy control and evaluation with respect to tumor edema as well as two-years survival was performed. Immunhistochemical staining of GBM tissue sections was conducted to depict AQP1 expression.

Results: Two single nucleotide polymorphisms (-783C > G/-781C > T) define 6 common haplotypes with altered reporter activity, allele specific transcription factor binding and AQP1 gene expression. The haplotype distribution in GBM is comparable to healthy control. At the two-years follow-up 122 (80.8%) of the 151 patients had died. Kaplan-Meier curves revealed significant differences in two-years survival according to the different haplotypes (p = 0.04), while there was no difference in peritumoral edema. Immunhistochemical staining revealed strong AQP1 expression on all GBM sections independent to the haplotype.

Conclusions: AQP1 promoter haplotypes might be predictive markers for survival of patients suffering from glioblastoma multiforme.