gms | German Medical Science

63rd Annual Meeting of the German Society of Neurosurgery (DGNC)
Joint Meeting with the Japanese Neurosurgical Society (JNS)

German Society of Neurosurgery (DGNC)

13 - 16 June 2012, Leipzig

Analysis of TGFB1 in European and Japanese Moyamoya disease patients

Meeting Abstract

  • C. Roder - Klinik für Neurochirurgie, Eberhard Karls Universität Tübingen, Tübingen
  • C. Liu - Klinik für Neurochirurgie, Eberhard Karls Universität Tübingen, Tübingen
  • C. Schulte - Hertie Institut für klinische Hirnforschung, Arbeitsgruppe Klinische Neurodegeneration und Deutsches Zentrum für Neurodegenerative Erkrankungen, Eberhard Karls Universität Tübingen, Tübingen
  • H. Kasuya - Department of Neurosurgery, Medical Center East, Tokyo Women’s Medical University, Tokyo, Japan
  • H. Akagawa - Tokyo Women's Medical University, Institute for Integrated Medical Sciences (TIIMS), Tokyo, Japan
  • T. Nishizawa - Division of Virology, Department of Infection and Immunity, Jichi Medical School, Shimotsuke, Japan
  • T. Yoneyama - Department of Neurosurgery, Medical Center East, Tokyo Women’s Medical University, Tokyo, Japan
  • Y. Okada - Department of Neurosurgery, Medical Center East, Tokyo Women’s Medical University, Tokyo, Japan
  • N. Khan - Moyamoya Klinik, Universitätskinderklinik Zürich, Zürich, Schweiz
  • M. Tatagiba - Klinik für Neurochirurgie, Eberhard Karls Universität Tübingen, Tübingen
  • D. Berg - Klinik für Neurologie, Abteilung Neurodegeneration, Eberhard Karls Universität Tübingen, Tübingen
  • B. Krischek - Klinik für Neurochirurgie, Eberhard Karls Universität Tübingen, Tübingen

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS). Leipzig, 13.-16.06.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. DocDO.15.12

DOI: 10.3205/12dgnc144, URN: urn:nbn:de:0183-12dgnc1442

Published: June 4, 2012

© 2012 Roder et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Objective: Despite large efforts in researching the genesis of Moyamoya disease (MMD), the etiology of this rare disease remains widely unknown. In a previous publication we described two genetic variants in the first exon of transforming growth factor beta 1 (TGFB1) which were associated and showed a tendency towards significance, respectively. In this study we performed a follow-up analysis of TGFB1 by sequencing the complete exon 1 in European and by genotyping previously described positively associated single nucleotide polymorphisms (SNPs) in Japanese patients with MMD.

Methods: The complete first exon of TGFB1 was genotyped in 40 MMD patients and 68 healthy controls from central Europe. For verification, genotyping of the previously described SNPs rs1800470 and rs1800471 was performed in 45 Japanese MMD patients and 79 healthy controls. Analysis was performed by capillary sequencing with custom made primers.

Results: Sequencing of the first exon of TGFB1 in the European cohort did not reveal any new disease-associated nor other genetic variations. The previously described disease association of rs1800471 and tendency towards significance of rs1800470 could not be replicated in the Japanese cohort.

Conclusions: As no new genetic variants were uncovered in this study of the first exon of TGFB1 in European MMD patients and because of the negative association of rs1800470 and rs1800471 in Japanese MMD patients, a role of this exon of TGFB1 in the genesis of MMD is unlikely. Further analyses with even larger cohorts may be necessary to detect causal genetic factors that contribute to the genesis of this disease.