gms | German Medical Science

61st Annual Meeting of the German Society of Neurosurgery (DGNC) as part of the Neurowoche 2010
Joint Meeting with the Brazilian Society of Neurosurgery on the 20 September 2010

German Society of Neurosurgery (DGNC)

21 - 25 September 2010, Mannheim

Multi-tract microtransplantation increases the yield of DARPP-32 positive embryonic striatal cells in a rodent model of Huntington's disease

Meeting Abstract

  • Fabian Büchele - Laboratory of Molecular Neurosurgery, Department of Stereotactic Neurosurgery, University Hospital Freiburg-Neurocentre, Freiburg, Germany
  • Wei Jiang - Department of Neurosurgery, Tongji Hospital of Huazhong University of Science and Technology, Wuhan, China
  • Anna Papazoglou - Laboratory of Molecular Neurosurgery, Department of Stereotactic Neurosurgery, University Hospital Freiburg-Neurocentre, Freiburg, Germany
  • Robert Kirch - Laboratory of Molecular Neurosurgery, Department of Stereotactic Neurosurgery, University Hospital Freiburg-Neurocentre, Freiburg, Germany
  • Máté Döbrössy - Laboratory of Molecular Neurosurgery, Department of Stereotactic Neurosurgery, University Hospital Freiburg-Neurocentre, Freiburg, Germany
  • Guido Nikkhah - Laboratory of Molecular Neurosurgery, Department of Stereotactic Neurosurgery, University Hospital Freiburg-Neurocentre, Freiburg, Germany

Deutsche Gesellschaft für Neurochirurgie. 61. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC) im Rahmen der Neurowoche 2010. Mannheim, 21.-25.09.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. DocP1888

DOI: 10.3205/10dgnc359, URN: urn:nbn:de:0183-10dgnc3591

Published: September 16, 2010

© 2010 Büchele et al.
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Outline

Text

Objective: Embryonic striatal tissue mediated functional recovery in the rodent lesion model of Huntington's disease (HD) correlates with the proportion of dopamine- and adenosine 3',5'-monophosphat-regulated phosphoprotein with a molecular weight of 32 kDa (DARPP-32) positive neurones in the graft. The current study investigated the impact of the microtransplantation procedure by comparing the DARPP-32 positive cell numbers in the grafts following either single-tract or multi-tract cell delivery protocols.

Methods: Cells derived from the whole ganglionic eminence of E15 rat embryos, ubiquitously expressing Green Fluorescent Protein (GFP), were implanted into unilaterally QA-lesioned rat striatum either as 2 x 1.8 μl deposits in a single-tract, or as 18 x 0.2 μl deposits disseminated over six needle, multi-tract, penetrations. For both groups, an ultra-thin glass capillary with an outer diameter of 50 μm was used.

Results: Histological assessment at 4 months after transplantation showed nearly two-fold increase of DARRP-32 positive striatal like neurons in the multi-tract compared to the single-tract group. Multi-tract grafts tended to have larger overall volumes, increased DARPP-32 positive zones and were more extensively innervated by dopaminergic projections. However, the cellular make-up of the grafts did not translate into functional differences as tested in simple spontaneous behaviour tests.

Conclusions: The results show that distribution of fetal striatal tissue in multiple submicroliter deposits provides for an increased yield of striatal-like neurons, potentially due to the enlargement of the graft-host border area intensifying the graft's exposure to host derived factors. Furthermore, the use of embryonic tissue from GFP donors was validated in cell based therapy studies in the HD model.